polyclonal antibodies against pept1 pept2  (Santa Cruz Biotechnology)

Journal: Animal Nutrition

Article Title: Dietary rumen-protected L-arginine or N-carbamylglutamate enhances placental amino acid transport and suppresses angiogenesis and steroid anabolism in underfed pregnant ewes

doi: 10.1016/j.aninu.2023.08.005

Figure Lengend Snippet: Effects of dietary N-carbamylglutamate (NCG) or rumen-protected L-arginine (RP-Arg) supplementation on the amino acid and peptide transporters-related protein expression in type A cotyledons (COT) tissues of underfed Hu ewes on d 110 of gestation . SLC7A1 , SLC1A5 , and SLC15A1 were responsible for encoding CAT1, ASCT2, and PEPT1, respectively. (A) Typical charts showing the Western blotting analysis. (B) Densitometric data were standardized using β-actin as a reference and presented as a relative change in the values. Results are expressed as means ± SEM ( n = 8/group for ewes, n = 16/group for the fetus). Means labelled with different letters indicate statistical significance ( P < 0.05). ASCT2 = alanine-serine-cysteine amino acid transporter 2; CON = ewes fed with 100% of nutrient requirements recommended by ; CAT1 = cationic amino acid transporter, y + system, member 1; PEPT1 = peptide transporter 1; RES = 50% of nutrient requirements recommended by ; RES + ARG = RES ewes supplemented with 20 g/d RP-Arg; RES + NCG = RES ewes supplemented with 5 g/d NCG.

Article Snippet: The following primary antibodies were used for WB assay: alanine-serine-cysteine AA transporter 2 (ASCT2) (1:500, A6981, ABclonal Technology, Wuhan, China); β-actin (1:1,000, AC006, ABclonal Technology, Wuhan, China); peptide transporter 1 (PEPT1) (1:500, bs-10588R, Bioss, Beijing, China); cationic AA transporter, y + system, member 1 (CAT1, 1:500, A14784, ABclonal Technology, Wuhan, China); progesterone receptor (PGR) (1:1000, AF7737, Beyotime Biotechnology, Shanghai, China); fibroblast growth factor 2 (FGF2) (1:500, bs-0217R, Bioss, Beijing, China); epithelial NOS (eNOS) (1:1,000, NB300-500, Novus, CO, USA).

Techniques: Expressing, Western Blot

Journal: Animal Nutrition

Article Title: Different copper sources and levels affect growth performance, copper content, carcass characteristics, intestinal microorganism and metabolism of finishing pigs

doi: 10.1016/j.aninu.2021.10.007

Figure Lengend Snippet: Effects of different copper sources and levels on the mRNA expression of CTR1 (A), ATP7A (B), ATOX1 (C), ASCT2 (D) and PepT1 (E) genes in jejunal mucosa. CuSO 4 = copper sulfate; Cu-Gly = copper glycinate; Cu-Pro = copper proteinate; CTR1 = high affinity copper uptake protein 1; ATP7A = ATPase copper transporting alpha; ATOX1 = Antioxidant 1 copper chaperone; ASCT2 = lanine-serine-cysteine transporter, type-2; PepT1 = peptide transporter 1. Data represent mean values ± standard error of the mean ( n = 6). Significant differences between processing treatments are represented by different lowercase letters ( P < 0.05).

Article Snippet: The membranes were blocked with 5% skim milk in Tris-buffered saline containing 0.05% Tween-20 (TBST) and incubated overnight at 4 °C with the antibodies: rabbit anti-CTR1 (1:1,000, Genetex, GTX30642), rabbit anti-PepT1 (1:1,000, Bioss, bs-0689R) and rabbit anti-actin (1:1,000, Absin, abs132001).

Techniques: Expressing

Journal: Animal Nutrition

Article Title: Different copper sources and levels affect growth performance, copper content, carcass characteristics, intestinal microorganism and metabolism of finishing pigs

doi: 10.1016/j.aninu.2021.10.007

Figure Lengend Snippet: Effects of different copper sources and levels on the mRNA Expression of CTR1 (A), ATP7A (B), ATP7B (C), ATOX1 (D), ASCT2 (E) and PepT1 (F) genes in liver. CuSO 4 = copper sulfate; Cu-Gly = copper glycinate; Cu-Pro = copper proteinate; CTR1 = high affinity copper uptake protein 1; ATP7A = ATPase copper transporting alpha; ATOX1 = Antioxidant 1 copper chaperone; ASCT2 = lanine-serine-cysteine transporter, type-2; PepT1 = peptide transporter 1. Data represent mean values ± standard error of the mean ( n = 6). Significant differences between processing treatments are represented by different lowercase letters ( P < 0.05).

Article Snippet: The membranes were blocked with 5% skim milk in Tris-buffered saline containing 0.05% Tween-20 (TBST) and incubated overnight at 4 °C with the antibodies: rabbit anti-CTR1 (1:1,000, Genetex, GTX30642), rabbit anti-PepT1 (1:1,000, Bioss, bs-0689R) and rabbit anti-actin (1:1,000, Absin, abs132001).

Techniques: Expressing

Journal: Animal Nutrition

Article Title: Different copper sources and levels affect growth performance, copper content, carcass characteristics, intestinal microorganism and metabolism of finishing pigs

doi: 10.1016/j.aninu.2021.10.007

Figure Lengend Snippet: Effects of different copper sources and levels on the protein expression for CTR1(A), and PepT1(B) in jejunal mucosa. CuSO 4 = copper sulfate; Cu-Gly = copper glycinate; Cu-Pro = copper proteinate; CTR1 = high affinity copper uptake protein 1; PepT1 = peptide transporter 1. Data represent mean values ± standard error of the mean ( n = 6). Significant differences between processing treatments are represented by different lowercase letters ( P < 0.05).

Article Snippet: The membranes were blocked with 5% skim milk in Tris-buffered saline containing 0.05% Tween-20 (TBST) and incubated overnight at 4 °C with the antibodies: rabbit anti-CTR1 (1:1,000, Genetex, GTX30642), rabbit anti-PepT1 (1:1,000, Bioss, bs-0689R) and rabbit anti-actin (1:1,000, Absin, abs132001).

Techniques: Expressing

Journal: Antioxidants

Article Title: The Cytotoxicity of Doxorubicin Can Be Accelerated by a Combination of Hyperthermia and 5-Aminolevulinic Acid

doi: 10.3390/antiox10101531

Figure Lengend Snippet: Western blot analysis of each transporter expression. SLC22A16 ( A , B ), ABCG2 ( C , D ) and SLC15A1 ( E , F ) were normalized to β-actin expression. Data are expressed as the mean ± SD ( n = 3). * p < 0.05, ** p < 0.01. Student’s t -test.

Article Snippet: Antirabbit SLC22A16 (cat. no. LS-C176922) (LSBio, Seattle, WA, USA), ABCG2 (cat. no. 42078) (Cell Signaling Technology Japan K.K., Tokyo, Japan), or SLC15A1 (cat. no. bs-10588R) (Bioss, Boston, MA, USA) antibodies (1:1000) were added to the Can Get Signal ® Immunoreaction Enhancer Solution 1 (Toyobo Co. Ltd., Osaka, Japan).

Techniques: Western Blot, Expressing